B

bacterial assembly

This repository contains a Snakemake workflow tailored for assembling bacterial genomes from long-read data generated with R10.4.1 simplex reads from Oxford Nanopore Technologies and includes several QC steps on the resulting assemblies.

A comprehensive Snakemake workflow for assembling high-quality bacterial genomes from Oxford Nanopore long-read sequencing data. The pipeline integrates Autocycler consensus assembly with multiple assemblers (Canu, Flye, Plassembler), performs quality control with Filtlong and Porechop, applies Racon polishing, and provides comprehensive quality assessment using QUAST, BUSCO, and CheckM2. All steps are containerized with conda environments for reproducible results. They include automated HTML/PDF/TXT reporting.

This repository contains a Snakemake bacterial assembly QC pipeline to perform quality control. It will use Quast (metrics), CheckM2 (completedness and contamination), Busco (completedness), skANI (taxonomic assignment against GTDB with ANI). It will also produce a Busco plot summary, a beeswarm plot of N50 and number of contigs, and an excel file with Quast, CheckM2 and skANI summaries.

Illumina bacterial assembly snakemake is a snakemake pipeline to assemble lots of bacterial genomes from Illumina paired-end short-reads (typically 150bp); It also performs several QC steps on the reads and on the resulting assemblies. Everything needed can be found in this repository (except for the GTDB reference database for taxonomic assembly classification and the Kraken2 database for taxonomical read classification).